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I figured out, and tried to delete the post, but no option for deleting.
The answer is: rowRanges(all)@elementMetadata has all information on the right hand side of bar separation.
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If I load a vcf file, and go to rowRanges slot:
> rowRanges(all)[2:5] GRanges object with 4 ranges and 5 metadata columns: seqnames ranges strand | paramRangeID REF <Rle> <IRanges> <Rle> | <factor> <DNAStringSet> 1:12783_G/A 1 [12783, 12783] * | <NA> G 1:13110_G/A 1 [13110, 13110] * | <NA> G 1:13116_T/G 1 [13116, 13116] * | <NA> T 1:13118_A/G 1 [13118, 13118] * | <NA> A ALT QUAL FILTER <DNAStringSetList> <numeric> <character> 1:12783_G/A A 33.56 . 1:13110_G/A A 103.15 . 1:13116_T/G G 1681.37 . 1:13118_A/G G 1591.21 .
I would like to take the values in the slot "REF", but in fact, this is not a slot:
> getClass(class(all@rowRanges)) Class "GRanges" [package "GenomicRanges"] Slots: Name: seqnames ranges strand elementMetadata Class: Rle IRanges Rle DataFrame Name: seqinfo metadata Class: Seqinfo list
I can get the information with names(rowRanges(all)), but some names in this vector are already translated to "rs" numbers.
So I still need to get values for the columns REF and ALT. How?
> sessionInfo() R version 3.3.3 (2017-03-06) Platform: x86_64-pc-linux-gnu (64-bit) Running under: openSUSE 13.1 (Bottle) (x86_64) locale: [1] LC_CTYPE=en_GB.UTF-8 LC_NUMERIC=C [3] LC_TIME=en_GB.UTF-8 LC_COLLATE=en_GB.UTF-8 [5] LC_MONETARY=en_GB.UTF-8 LC_MESSAGES=en_GB.UTF-8 [7] LC_PAPER=en_GB.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=en_GB.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] stats4 parallel stats graphics grDevices utils datasets [8] methods base other attached packages: [1] VariantAnnotation_1.20.3 Rsamtools_1.26.2 [3] Biostrings_2.42.1 XVector_0.14.1 [5] SummarizedExperiment_1.4.0 Biobase_2.34.0 [7] GenomicRanges_1.26.4 GenomeInfoDb_1.10.3 [9] IRanges_2.8.2 S4Vectors_0.12.2 [11] BiocGenerics_0.20.0 loaded via a namespace (and not attached): [1] Rcpp_0.12.10 AnnotationDbi_1.36.2 GenomicAlignments_1.10.1 [4] zlibbioc_1.20.0 BiocParallel_1.8.2 BSgenome_1.42.0 [7] lattice_0.20-35 tools_3.3.3 grid_3.3.3 [10] DBI_0.6-1 digest_0.6.12 Matrix_1.2-8 [13] rtracklayer_1.34.2 bitops_1.0-6 biomaRt_2.30.0 [16] RCurl_1.95-4.8 memoise_1.1.0 RSQLite_1.1-2 [19] GenomicFeatures_1.26.4 XML_3.98-1.6