Entering edit mode
jon.barenboim
•
0
@jonbarenboim-12542
Last seen 7.8 years ago
I am using bumphunters' matchGenes function to annotate some genomic data, and am getting this error when some regions cannot be mapped with `nearest`
Error in NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) : subscript contains NAs or out-of-bounds indices
The code I am running is below:
>library(GenomicFeatures) >library(bumphunter) >dmrs <- read.csv(path/to/file) >library(TxDb.Hsapiens.UCSC.hg19.knownGene) >txdb <- TxDb.Hsapiens.UCSC.hg19.knownGene >annotations <- annotateTranscripts(txdb) >matched <- matchGenes(dmrs, annotations) Error in NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) : subscript contains NAs or out-of-bounds indices 9: stop("subscript contains NAs or out-of-bounds indices") 8: NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) 7: NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) 6: normalizeSingleBracketSubscript(i, x) 5: extractROWS(x, i) 4: extractROWS(x, i) 3: subject[i, ] 2: subject[i, ] 1: matchGenes(dmrs, annotations)
The error only occurs when `nearest` fails to map some regions
> map <- nearest(makeGRangesFromDataFrame(dmrs), annotations) > which(is.na(map))[1:10] [1] 729 1379 1624 1927 1973 1986 1987 2031 2099 2111 > a <- matchGenes(dmrs[700:725,], annotations) > b <- matchGenes(dmrs[725:730,], annotations) Error in NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) : subscript contains NAs or out-of-bounds indices 9: stop("subscript contains NAs or out-of-bounds indices") 8: NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) 7: NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) 6: normalizeSingleBracketSubscript(i, x) 5: extractROWS(x, i) 4: extractROWS(x, i) 3: subject[i, ] 2: subject[i, ] 1: matchGenes(dmrs[725:730, ], annotations)
The error disappears when the first line of the for loop in matchGenes is changed from
i <- map[ind][j]
to
i <- map[j]
dmrs is a dataframe of regions obtained from bumphunterEngine. The first line is
> dmrs[1,] chr start end value area cluster indexStart indexEnd L clusterL p.value fwer p.valueArea fwerArea 1 chr19 48945971 48947362 -0.5952619 19.64364 30722 467311 467396 86 98 1.804523e-06 0.1 1.804523e-06 0.1
(The relevant part of) my session info is:
R version 3.2.3 (2015-12-10) Platform: x86_64-pc-linux-gnu (64-bit) Running under: CentOS release 6.8 (Final) other attached packages: [1] org.Hs.eg.db_3.2.3 RSQLite_1.0.0 DBI_0.3.1 [4] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2 bumphunter_1.10.0 locfit_1.5-9.1 [7] iterators_1.0.8 foreach_1.4.3 GenomicFeatures_1.22.13 [10] AnnotationDbi_1.32.3 Biobase_2.30.0 GenomicRanges_1.22.4 [13] GenomeInfoDb_1.6.3 IRanges_2.4.8 S4Vectors_0.8.11 [16] BiocGenerics_0.16.1
Hi,
You seem to be using Bioconductor 3.2 which is not supported anymore. Please update your installation to use the current version of Bioconductor (BioC 3.4). It requires R 3.3 which is also the most current release of R.
Thanks,
H.