Hi,
I am trying to follow the workflow from the hemberg lab about single-cell analysis. There is though a conflict in the versions.
I try to run the SC3
package. There is a function here called sc3_prepare
, but under my R environment it doesn't seem to exist.
I keep getting the error message (running the script in the file 17-clustering.Rmd)
Quitting from lines 94-95 (17-clustering.Rmd) Error in eval(expr, envir, enclos) : could not find function "sc3_prepare" Calls: <Anonymous> ... handle -> withCallingHandlers -> withVisible -> eval -> eval In addition: Warning messages: 1: package 'mclust' was built under R version 3.3.2 2: package 'ggplot2' was built under R version 3.3.2 Execution halted
looking at my sc3 package i can't find this function at all.
is there something i am doing wrong here?
thanks
Assa
> sessionInfo() R version 3.3.0 (2016-05-03) Platform: x86_64-apple-darwin13.4.0 (64-bit) Running under: OS X 10.11.6 (El Capitan) locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base packages: [1] parallel stats graphics grDevices utils datasets methods base other attached packages: [1] SC3_1.1.4 scater_1.0.4 ggplot2_2.2.1 Biobase_2.32.0 BiocGenerics_0.18.0 [6] BiocInstaller_1.22.3 loaded via a namespace (and not attached): [1] bitops_1.0-6 matrixStats_0.51.0 doParallel_1.0.10 RColorBrewer_1.1-2 rprojroot_1.2 [6] tools_3.3.0 backports_1.0.5 doRNG_1.6 R6_2.2.0 KernSmooth_2.23-15 [11] DBI_0.5-1 lazyeval_0.2.0 colorspace_1.3-2 gridExtra_2.2.1 pkgmaker_0.22 [16] bookdown_0.3 caTools_1.17.1 scales_0.4.1 DEoptimR_1.0-8 mvtnorm_1.0-5 [21] robustbase_0.92-7 stringr_1.1.0 digest_0.6.12 rmarkdown_1.3 wdman_0.2.2 [26] rrcov_1.4-3 htmltools_0.3.5 WriteXLS_4.0.0 limma_3.28.21 RSQLite_1.1-2 [31] shiny_1.0.0 gtools_3.5.0 dplyr_0.5.0 RCurl_1.95-4.8 magrittr_1.5 [36] Rcpp_0.12.9 munsell_0.4.3 S4Vectors_0.10.3 viridis_0.3.4 stringi_1.1.2 [41] yaml_2.1.14 edgeR_3.14.0 zlibbioc_1.18.0 rhdf5_2.16.0 gplots_3.0.1 [46] Rtsne_0.11 plyr_1.8.4 grid_3.3.0 gdata_2.17.0 shinydashboard_0.5.3 [51] semver_0.2.0 lattice_0.20-34 knitr_1.15.1 rjson_0.2.15 rngtools_1.2.4 [56] reshape2_1.4.2 codetools_0.2-15 biomaRt_2.28.0 stats4_3.3.0 XML_3.98-1.5 [61] evaluate_0.10 data.table_1.10.4 httpuv_1.3.3 foreach_1.4.3 gtable_0.2.0 [66] openssl_0.9.6 assertthat_0.1 binman_0.1.0 mime_0.5 xtable_1.8-2 [71] e1071_1.6-8 rsconnect_0.7 class_7.3-14 pcaPP_1.9-61 tibble_1.2 [76] pheatmap_1.0.8 iterators_1.0.8 RSelenium_1.7.1 AnnotationDbi_1.34.4 registry_0.3 [81] memoise_1.0.0 IRanges_2.6.1 tximport_1.0.3 cluster_2.0.5 ROCR_1.0-7
I have updated the SC3 package to 1.3.14.
Now I get a different error message, which is even before the
sc3_prepare
error.when running this command
I get the following error
thanks again
Assa
P.S.
but at least I can now see the sc3_prepare function, so it probably exists, when I get there.
thanks I have updated R to 3.3.2 and bio to 3.4. now it works. I do get a different error message, but this seems to be something else.
when running the jackStraw function it throws back an error -
I do need to take a closer look at Seurat though.
Note that in the course we used an old version of Seurat (1.3). Now there is a new one available, where the clustering algorithm is completely different. We will try to update the course with this new version when we run it next time (middle of March 2017).