Hello, Is it possible to normalise Affymetrix chips with MAS 4.0 in BioC/R? I would like to reproduce the results in a study. The data has been preprocessed with MAS 4. In the affy-package MAS 5 has been implemented as mas5(). Is there anything like that available for MAS 4? Thanks, Axel -- ******************************************* Dipl. Math. ETH Axel Rasche Max-Planck-Institute for Molecular Genetics Department Lehrach (Vertebrate Genomics) Ihnestrasse 63-73 D-14195 Berlin-Dahlem GERMANY Tel. ++49-30-8413-1289 Fax ++49-30-8413-1380

Axel Rasche wrote: > Hello, > > Is it possible to normalise Affymetrix chips with MAS 4.0 in BioC/R? > > I would like to reproduce the results in a study. The data has been > preprocessed with MAS 4. In the affy-package MAS 5 has been implemented > as mas5(). Is there anything like that available for MAS 4? Not that I know of. However, IIRC, MAS4 was simply the average difference, so it would be relatively simple for you to calculate those values yourself. Best, Jim > > Thanks, > Axel > -- James W. MacDonald Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623