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Hi,
I've been using the TPP package in R & it has been working great. I noticed not too long ago that the curve fitting might be different between the TPP TR results excel that gets generated and the melt curve pdf with the table of reported values underneath. This doesn't happen for every protein but sometimes there are slight differences which to me looks like potential differences in how the curves are fitted. I made sure that the TrReqs are the same for each of the lines I run for either the results excel + QC plots (analyzeTPPTR) & for the tpptrCurveFit generating line.
You will need to provide a self-contained example, or it is unlikely that anybody could debug any possible issue.