Problems with GSEA
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Sidra.Gull • 0
@c292dc7d
Last seen 13 hours ago
Germany

hallo,

I want to re-run the GSEA on my same data. I have previously performed the compare cluster analysis for GSEGSEA and it worked very well but now I have updated the R version to 4.4.2. here is the pathway and code with error. Kindly let me know what the issue is now

.
set.seed(1)

.....ck_TP_VL_HM<-compareCluster(geneClusters = geneList_TP_VL, fun="GSEA",TERM2GENE = hallmark_gs,

                        pAdjustMethod="fdr", 
                        seed=TRUE, by="fgsea")
Fehler in .stopf("Duplicate values in %s not allowed", universeArg) :
Duplicate values in names(stats) not allowed
Zusätzlich: Warnmeldung:
In preparePathwaysAndStats(pathways, stats, minSize, maxSize, gseaParam, :
There are ties in the preranked stats (10.73% of the list).
The order of those tied genes will be arbitrary, which may produce unexpected results.
GSEABase • 61 views
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Guido Hooiveld ★ 4.1k
@guido-hooiveld-2020
Last seen 4 hours ago
Wageningen University, Wageningen, the …

The error Duplicate values in names(stats) not allowed (from fgsea that is used under the hood) shows that the input is not unique; in other words some genes are present multiple times. This often happens when gene symbols are used as identifiers.

Also cross-posted: https://github.com/YuLab-SMU/clusterProfiler/issues/754

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