Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
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@annacotannacot-20795
Last seen 7 hours ago
United States

I have two RNA-seq experiments performed in different batches several months apart. Both experiments include technical replicates of the same 10 samples from condition A (with identical sample names: A1, A2, ..., A10). Exactly same control samples were used.

Experiment 1: 10 samples from condition A and 10 samples from condition B.

Experiment 2: 10 samples from condition A and 10 samples from condition C.

How can I use the technical replicates (condition A) to normalize for batch effects and perform differential gene expression analysis between conditions B and C?

I have processed my reads with kallisto so far and tximport.

I appreciate all your help!

limma DESeq2 edgeR • 25 views
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