DESeq2 interaction between cancer cells and microenvironment cells
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@5f0ace68
Last seen 27 minutes ago
United Kingdom

Hi, I am running a DESeq2 analysis which includes an interaction term between treated_cells and contamination (contamination = 1- purity ). The DESeq design looks like this :

dds <- DESeqDataSetFromMatrix( countData = counts, colData = manifest, design = ~ contamination + treated_cells + contamination : treated_cells )

With contamination and treated_cells being two continuous variables.

My question is: being interested in the interaction between contamination and treated_cells, is there a way to discriminate down or upregulated genes that likely come from the contamination (or treated_cells) ?

Many thanks, Selma.

DESeq2 • 25 views
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