How should I know which platforms are available for TCGA methylation?
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0
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@xiaofeiwang198266-13657
Last seen 20 hours ago
United States

As the code block here , screenshot below.

It worked to get data from platform of Illumina Human Methylation 27 for TCGA-B6-A0IM. But, when I tried to change the platform argument to "Illumina Methylation Epic", it gave me an error as below. I assumed Epic may not be available. Firstly, I thought it may relate to the packages version. But, the error is still there after updating TCGAbiolinks to the lastest one.

So, my question is how should I know the platform availability for TCGA? Thanks!

 query_met.hg38 <- GDCquery(
     project = "TCGA-BRCA", 
     data.category = "DNA Methylation", 
     data.type = "Methylation Beta Value",
     platform = "Illumina Methylation Epic", 
     barcode = c("TCGA-B6-A0IM")
 )


# include your problematic code here with any corresponding output 

--------------------------------------
o GDCquery: Searching in GDC database
--------------------------------------
Genome of reference: hg38
--------------------------------------------
oo Accessing GDC. This might take a while...
--------------------------------------------
ooo Project: TCGA-BRCA
--------------------
oo Filtering results
--------------------
Error in GDCquery(project = "TCGA-BRCA", data.category = "DNA Methylation",  : 
  Please set a valid platform argument from the list below:
  => Illumina Human Methylation 27
  => Illumina Human Methylation 450


other attached packages:
 [1] GenomicRanges_1.56.2 GenomeInfoDb_1.40.1  IRanges_2.38.1       S4Vectors_0.42.1     sesameData_1.22.0    ExperimentHub_2.12.0
 [7] AnnotationHub_3.12.0 BiocFileCache_2.12.0 dbplyr_2.5.0         BiocGenerics_0.50.0  TCGAbiolinks_2.32.0 

loaded via a namespace (and not attached):
 [1] DBI_1.2.3                   httr2_1.0.5                 biomaRt_2.60.1              rlang_1.1.4                
 [5] magrittr_2.0.3              matrixStats_1.4.1           compiler_4.4.1              RSQLite_2.3.7              
 [9] reshape2_1.4.4              png_0.1-8                   vctrs_0.6.5                 rvest_1.0.4                
[13] stringr_1.5.1               pkgconfig_2.0.3             crayon_1.5.3                fastmap_1.2.0              
[17] XVector_0.44.0              utf8_1.2.4                  tzdb_0.4.0                  preprocessCore_1.66.0      
[21] UCSC.utils_1.0.0            purrr_1.0.2                 bit_4.5.0                   xfun_0.48                  
[25] zlibbioc_1.50.0             cachem_1.1.0                jsonlite_1.8.9              progress_1.2.3             
[29] blob_1.2.4                  DelayedArray_0.30.1         BiocParallel_1.38.0         parallel_4.4.1             
[33] prettyunits_1.2.0           R6_2.5.1                    stringi_1.8.4               RColorBrewer_1.1-3         
[37] sesame_1.22.2               Rcpp_1.0.13                 SummarizedExperiment_1.34.0 knitr_1.48                 
[41] wheatmap_0.2.0              downloader_0.4              R.utils_2.12.3              readr_2.1.5                
[45] Matrix_1.7-0                tidyselect_1.2.1            abind_1.4-8                 yaml_2.3.10                
[49] codetools_0.2-20            curl_5.2.3                  lattice_0.22-6              tibble_3.2.1               
[53] plyr_1.8.9                  Biobase_2.64.0              withr_3.0.1                 KEGGREST_1.44.1            
[57] xml2_1.3.6                  Biostrings_2.72.1           pillar_1.9.0                BiocManager_1.30.25        
[61] filelock_1.0.3              MatrixGenerics_1.16.0       generics_0.1.3              BiocVersion_3.19.1         
[65] hms_1.1.3                   ggplot2_3.5.1               munsell_0.5.1               scales_1.3.0               
[69] glue_1.8.0                  tools_4.4.1                 data.table_1.16.2           XML_3.99-0.17              
[73] grid_4.4.1                  tidyr_1.3.1                 AnnotationDbi_1.66.0        colorspace_2.1-1           
[77] GenomeInfoDbData_1.2.12     cli_3.6.3                   rappdirs_0.3.3              fansi_1.0.6                
[81] S4Arrays_1.4.1              dplyr_1.1.4                 gtable_0.3.5                R.methodsS3_1.8.2          
[85] TCGAbiolinksGUI.data_1.24.0 digest_0.6.37               SparseArray_1.4.8           memoise_2.0.1              
[89] R.oo_1.26.0                 lifecycle_1.0.4             httr_1.4.7                  mime_0.12                  
[93] bit64_4.5.2

enter image description here

TCGAbiolinks • 873 views
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@james-w-macdonald-5106
Last seen 1 day ago
United States

Doesn't this tell you which platforms are available?

Error in GDCquery(project = "TCGA-BRCA", data.category = "DNA Methylation",  : 
  Please set a valid platform argument from the list below:
  => Illumina Human Methylation 27
  => Illumina Human Methylation 450
0
Entering edit mode

Yes, it does. But, I mean how should I know in advance or where can I find the information before running code. Because I have several projects which need downloading. I'd like to avoid the error in my loop. Sorry for the confusion.

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I don't see any evidence that TCGA has any EPIC data, but there may be some. Going to their webpage is an exercise in frustration and I didn't try too hard to figure out how it's meant to work. But anyway, you might just add a tryCatchWE (https://search.r-project.org/CRAN/refmans/simsalapar/html/tryCatchWE.html) to your code to continue in the face of errors.

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Thanks a lot!

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Sorry to get another error.

I want to download it in hg19, I used the code as below. It looks like legacy = TRUE does not work.

>  query.16meth.ls[[i]] <- GDCquery(
+      project = paste("TCGA-", names(aliquots_id_ls)[i], sep=""),
+      data.category = "DNA Methylation", 
+      data.type = "Methylation Beta Value",
+      platform = "Illumina Human Methylation 450", 
+      legacy = TRUE,
+      barcode = aliquots_id_ls[[i]]$submitter_id
+      )
Error in GDCquery(project = paste("TCGA-", names(aliquots_id_ls)[i], sep = ""),  : 
  unused argument (legacy = TRUE)
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Hi @James W. macDonald, I want to check if "unused argument (legacy = TRUE)" due to the GDC legacy archive retirement?

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There is a NEWS file for almost all packages that provides, uh, news about the package. You can always read it on the landing page for the package.

Or you can read it directly in R

library(TCGAbiolinks)
> readLines(paste0(path.package("TCGAbiolinks"), "/NEWS"), 10)
 [1] "CHANGES IN VERSION 2.29.1"                                                        
 [2] "-------------------------"                                                        
 [3] ""                                                                                 
 [4] "* Removing support to legacy archive since it will be shutdown by GDC soon."      
 [5] "* When saving files we will not include folders prefix  legacy/harmonized anymore"
 [6] ""                                                                                 
 [7] "CHANGES IN VERSION 2.21.1"                                                        
 [8] "-------------------------"                                                        
 [9] ""                                                                                 
[10] "* Function GDCPrepare for TARGET-ALL-P3 fixed"

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