Cannot connect to annotationnhhub server Code should be placed in three backticks as shown below

ah <- AnnotationHub(localHub = FALSE)

# Cannot connect to AnnotationHub server, using 'localHub=TRUE' instead using 'localHub=TRUE' updateHubDB(hub_bfc, .class, url, proxy, localhub):
Invalid cache: sqlite file 
Hub has not been added to cache 
Run again with 'localhub=FALSE' 

sessionInfo( )

R version 4.4.0 (2024-04-24 ucrt)
Platform: x86_64-w64-mingw32/x64
Running under: Windows 10 x64 (build 19045)

Matrix products: default


locale:
[1] LC_COLLATE=English_United States.utf8  LC_CTYPE=English_United States.utf8    LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C                           LC_TIME=English_United States.utf8    

time zone: Asia/Shanghai
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] pheatmap_1.0.12             sesame_1.23.6               sesameData_1.23.0           ExperimentHub_2.13.0        maftools_2.21.0            
 [6] lubridate_1.9.3             forcats_1.0.0               stringr_1.5.1               dplyr_1.1.4                 purrr_1.0.2                
[11] readr_2.1.5                 tidyr_1.3.1                 tibble_3.2.1                ggplot2_3.5.1               tidyverse_2.0.0            
[16] TCGAbiolinks_2.33.0         zlibbioc_1.51.1             XVector_0.45.0              S4Arrays_1.5.1              abind_1.4-5                
[21] Matrix_1.7-0                SummarizedExperiment_1.35.0 Biobase_2.65.0              GenomicRanges_1.57.1        GenomeInfoDb_1.40.1        
[26] IRanges_2.38.0              S4Vectors_0.42.0            MatrixGenerics_1.16.0       matrixStats_1.3.0           AnnotationHub_3.13.0       
[31] BiocFileCache_2.13.0        dbplyr_2.5.0                BiocGenerics_0.50.0        

loaded via a namespace (and not attached):
 [1] DBI_1.2.3                   httr2_1.0.1                 biomaRt_2.61.1              rlang_1.1.4                 magrittr_2.0.3             
 [6] compiler_4.4.0              RSQLite_2.3.7               reshape2_1.4.4              png_0.1-8                   vctrs_0.6.5                
[11] rvest_1.0.4                 pkgconfig_2.0.3             crayon_1.5.2                fastmap_1.2.0               utf8_1.2.4                 
[16] tzdb_0.4.0                  preprocessCore_1.67.0       UCSC.utils_1.1.0            bit_4.0.5                   xfun_0.45                  
[21] cachem_1.1.0                jsonlite_1.8.8              progress_1.2.3              blob_1.2.4                  DelayedArray_0.31.1        
[26] BiocParallel_1.39.0         parallel_4.4.0              prettyunits_1.2.0           R6_2.5.1                    stringi_1.8.4              
[31] RColorBrewer_1.1-3          DNAcopy_1.79.0              Rcpp_1.0.12                 knitr_1.47                  wheatmap_0.2.0             
[36] downloader_0.4              splines_4.4.0               timechange_0.3.0            tidyselect_1.2.1            rstudioapi_0.16.0          
[41] yaml_2.3.8                  codetools_0.2-20            curl_5.2.1                  lattice_0.22-6              plyr_1.8.9                 
[46] withr_3.0.0                 KEGGREST_1.45.1             survival_3.7-0              xml2_1.3.6                  Biostrings_2.73.1          
[51] pillar_1.9.0                BiocManager_1.30.23         filelock_1.0.3              generics_0.1.3              BiocVersion_3.20.0         
[56] hms_1.1.3                   munsell_0.5.1               scales_1.3.0                glue_1.7.0                  tools_4.4.0                
[61] data.table_1.15.4           XML_3.99-0.16.1             grid_4.4.0                  AnnotationDbi_1.67.0        colorspace_2.1-0           
[66] GenomeInfoDbData_1.2.12     cli_3.6.2                   rappdirs_0.3.3              fansi_1.0.6                 gtable_0.3.5               
[71] TCGAbiolinksGUI.data_1.25.0 digest_0.6.35               SparseArray_1.5.8           memoise_2.0.1               lifecycle_1.0.4            
[76] httr_1.4.7                  bit64_4.0.5

Do you have a strong internet connect and/or are you behind a proxy ?

Yes my internet connection is fine, I have checked it and also i am not behind any proxy.

AnnotationHub::getAnnotationHubOption("CACHE")

[1] "C:\Users\Administrator\AppData\Local/R/cache/R/AnnotationHub"

library(AnnotationHub)

hub=getAnnotationHubOption("URL")

readBin(hub,n=1L,what = "raw")

Error in file(con, "rb"):

cannot open the connection to 'https://annotationhub.bioconductor.org'

Additionally: Warning message

In file(con, "rb") :

URL 'https://annotationhub.bioconductor.org/': Timeout of 60 seconds was reached