Hi

I was wondering if there is any way to extract DEGs from monocle pseudotime trajectory. I want to extract DEGs for a particular pseudotime state vs all others. Is there any way to do so?

Thanks!

You might try the methods in this paper:

Cheng J, Smyth GK, Chen Y (2023). Unraveling the timeline of gene expression: A pseudotemporal trajectory analysis of single-cell RNA sequencing data (version 2). F1000Research 12, 684.