Hello, everyone. There is a warning message while I use lfcShrink function. I wanna know why this warning message occured?

In nbinomGLM(x = x, Y = YNZ, size = size, weights = weightsNZ, offset = offsetNZ, : the line search routine failed, unable to sufficiently decrease the function value

This happens some time. You can use type="ashr" instead.

See the posting guide about how to include example code when posting to the support site.

https://bioconductor.org/help/support/posting-guide/