Hello,

I have a quick question regarding using the r packages "rain" to analyze my temporal microbiome dataset.However, I keep getting an error stating "invalid annotation of time points."

I am not sure why I keep getting this error, if its a matrix formatting issue or what exactly is happening. If someone can please help me out, I would greatly appreciate it.

My data format

• Data for 11 years (2012-2022, with no data for 2013 ).
• Data seasonally (4x/year for 11 years), but some years missing some seasons
• Replicates vary per season/yr

Data structure for rain = irregular time series (since I am missing data for 2012), and irregular repeats

Data matrix names are are sites x asv ID

• 2012_Q1_1 (replicate 1 for year 2012)
• 2012_Q2_2 (replicate 2 for year 2012)
• 2014_Q1_1 (replicate 1 for year 2014)
• 2014_Q1_2 (replicate 2 for year 2014)
• 2014_Q1_3 (replicate 3 for year 2014)

So overall the replicates should be

• measure.sequence=c(4,0,4,17,7,8,11,30,25,25,35)
• period= I put 11 since its 11 years but not sure
• deltat= 1 *sampling int = 1 per year or should it be 4 since we sampled 4x/yr

output_RAIN<-rain(DFdetrend, method="longitudinal", deltat=1, period = 11, measure.sequence=c(4, 0, 4, 17,7,8,11,30,25,25,35), na.rm = TRUE)

Error in rain(DFdetrend, method = "longitudinal", measure.sequence = c(4,  : 
  invalid annotation of time points