Hi, I am a totally new to R-studio and it's software. I have been trying to follow the tutorial posted on "https://stephenturner.github.io/deseq-to-fgsea/#load_some_results_from_deseq2"

I had used the using FGSEA package on, "https://bioconductor.org/packages/release/bioc/html/fgsea.html" and had followed the Installation for R.

1: Is there a more detail and step by step tutorial for this. I had found some but they required some knowledge of coding? 2: How do I import my own data into the software so the GSEA is shown as in website 1. 3: Here is an example of my data for GSEA:

#1.2                            
545 6                       
NAME    description WTcontrol1  WTcontrol2  WTcontrol3  WTDTnormalized1 WTDTnormalized2 WTDTnormalized3
ENSMUSG00000071103  1700029J07Rik   -0.2826271  0.03396225  -0.008006096    0.3456192   0.43789434  0.49301577
ENSMUSG00000100573  1700081H04Rik   -0.002503395    -0.01300478 0.002502918 -0.084319115    -0.0729208  -0.05217266
ENSMUSG00000097162  2310010J17Rik   -0.031499863    -0.18440628 -0.04216671 0.16484451  0.18860006  0.13092566
ENSMUSG00000032062  2310030G06Rik   -0.23690319 -0.21158266 -0.102458   0.16413021  0.24428272  0.31841516
ENSMUSG00000072852  2310040G07Rik   -0.03087902 -0.15501404 -0.079369545    0.268198    0.17418766  0.1296587

You don't want to use any 'normalized' data as input to DESeq2. Instead you want the raw counts/gene. There is a vignette for DESeq2 that you can read, as well as a vignette for fgsea, both of which are tutorials that are meant to help people get started.