nondetects and technical replicates
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Ed Siefker ▴ 230
@ed-siefker-5136
Last seen 12 months ago
United States

I'm trying to wrap my head around the nondetects package.

Looking at the oncogene2013 data set, they say they used 2 cell lines, three treatments, and 4 replicates. That's 24 samples, which is the number of columns in exprs().

What's not discussed is the number of technical replicates, which I assume they must have done.

Can nondetects impute a dataset that includes technical replicates? Or do you just toss the replicates that don't work and use the geometric mean of anything remaining?

Thanks -Ed

qPCR nondetects • 415 views
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