Long-read mode in featureCounts
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mmisak • 0
@6a9a6067
Last seen 12 months ago
Germany

Hello, I have a rather simple question: featureCounts has a long-read mode ("isLongRead" parameter). How does this parameter affect the counting behavior of featureCounts?

Thank you

Rsubread LongRead • 1.0k views
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Wei Shi ★ 3.6k
@wei-shi-2183
Last seen 11 weeks ago
Australia/Melbourne/Olivia Newton-John …

It doesn't really change the behaviour of featureCounts. It just instructs featureCounts to consider more exons and exon junctions in a long read.

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Thank you for your reply.

So does that mean, it impacts only the behavior of the "juncCounts" (-J) parameter and not at all the counting on meta-feature (i.e. gene) level or anything else? If so, to how many junctions is featureCounts limited in normal (i.e. short read) mode and how many are supported when setting the "isLongRead" (-L) parameter? I tried to find this in the user's guide but couldn't find any information on it.

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