Hi everyone,

I have the scRNA-seq data of patient samples comprising immune and cancer cells. I would like to segregate the cancer cells into 2 groups (low vs high) based on their level of expression of Gene X (median cut off), followed by DEG analysis between these 2 groups.

How should I go about it for single-cell RNA-seq analysis in R? Is there a need for pseudobulk analysis?

Thank you so much.

The support site is for technical help with the packages. Such question is better suited at biostars.org and even there it is expected that you do some sort of preliminary effort or provide toy data that can people use to help. As for now, you're essentially asking for spoon-feeding.