Create design matrix for differential methylation analysis
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titangerd • 0
@338bb3b1
Last seen 3 days ago
Germany

I´m working in R with a data frame of 32 Samples that contains m values from Infinium EPIC . I want to do differential methylation analysis with the command "cpg.annotate()" and therefore need an design matrix.

So far I Followed this Instruction: https://www.bioconductor.org/packages/release/workflows/vignettes/methylationArrayAnalysis/inst/doc/methylationArrayAnalysis.html

Unfortunately its not described in detail how you make a design matrix and a cont.matrix

From the data frame I want to compare 12 Samples (with Exposure) to 8 Samples (without Exposure)

Can anybody help me to make a design matrix or show how it should look?

screenshot of a part of the data frame

Annotation annotate DifferentialMethylation minfiDataEPIC • 754 views
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Basti ▴ 780
@7d45153c
Last seen 1 day ago
France

The design matrix creation is described part 2.7, please do not skip parts in the tutorial

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