I'm interested in utilizing the NOIseq R package for performing differential expression analysis, specifically focusing on a scenario where one group has replicates while the other does not. I would like to generate a comparison between these two groups and understand whether it's possible to conduct this type of analysis using the NOIseq package.

I'm wondering if the NOIseq package supports this kind of analysis where one group has replicates and the other does not. Can I perform a comparative analysis between these two groups and obtain meaningful results using NOIseq? If yes, could you kindly provide some guidance on how to set up the analysis or direct me to relevant resources in the package documentation?

Please read the manual. These very basics are addressed already on page three of the user guide in section "2.2 Factors".

https://bioconductor.org/packages/release/bioc/vignettes/NOISeq/inst/doc/NOISeq.pdf

Note that with replication in at least one group you can also use any of the other prominent differential expression tools such as edgeR, limma and DESeq2.