flowAI Error: Unable to Allocate Vector Size with FCS Exported from flowJo
0
0
Entering edit mode
Simoluc • 0
@17ec8f53
Last seen 15 months ago
Italy

Hi!

I'm experiencing issues while using the flowAI package in R.

When I use FCS files obtained directly from the LSRFortessa instrument, I have no problems with flowAI. However, when I try to use flowAI on FCS files exported from flowJo (for example: FCS files from which I extracted only B cell events), I encounter this error using the function flow_auto_qc:


>fs #fs is my flowSet object
A flowSet with 283 experiments.

column names(22): FSC-A FSC-H ... FJComp-PerCP-Cy5-5-A Time

>fs.ai<-flow_auto_qc(fs, remove_from = "FS_FM")
Quality control for the file: AAM_d7_CD19 +
'Error: cannot allocate vector of size 856.3 Gb.'

Do you know if there are compatibility issues between flowJo and flowAI?

I have also used the flowAI plugin in flowJo, using the latest versions of the plugin and flowAI and flowCore packages, and it has always worked fine. However, it doesn't work in R. Do you have any advice or suggestions? Thank you.

I am using these package versions: flowAI v1.30.0, flowCore v2.12.2, R version 4.3.1.

flowAI • 500 views
ADD COMMENT

Login before adding your answer.

Traffic: 645 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6