ggtree functions suddenly stopped working: "Error in offspring.tbl_tree_item(..."
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0
Entering edit mode
Hank • 0
@32a0ec3b
Last seen 16 months ago
United States

I am fairly new to ggtree and it had been working until today.

Today, several functions stopped working:

-- MRCA(), geom_hilight(), and geom_cladelab()

I have searched the web for answers.

Example:

> sessionInfo( )
R version 4.3.1 (2023-06-16)
Platform: x86_64-apple-darwin20 (64-bit)
Running under: macOS Ventura 13.4.1

Matrix products: default
BLAS:   /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib 
LAPACK: /Library/Frameworks/R.framework/Versions/4.3-x86_64/Resources/lib/libRlapack.dylib;  LAPACK version 3.11.0

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: America/Chicago
tzcode source: internal

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] ape_5.7-1      tidytree_0.4.4 ggtree_3.8.0  

loaded via a namespace (and not attached):
 [1] gtable_0.3.3        jsonlite_1.8.7      BiocManager_1.30.21 dplyr_1.1.2        
 [5] compiler_4.3.1      tidyselect_1.2.0    aplot_0.1.10        Rcpp_1.0.11        
 [9] gridGraphics_0.5-1  parallel_4.3.1      ggplotify_0.1.1     tidyr_1.3.0        
[13] ggfun_0.1.1         scales_1.2.1        lattice_0.21-8      ggplot2_3.4.2      
[17] R6_2.5.1            labeling_0.4.2      generics_0.1.3      patchwork_1.1.2    
[21] yulab.utils_0.0.6   tibble_3.2.1        munsell_0.5.0       pillar_1.9.0       
[25] rlang_1.1.1         treeio_1.24.2       utf8_1.2.3          lazyeval_0.2.2     
[29] cli_3.6.1           withr_2.5.0         magrittr_2.0.3      digest_0.6.33      
[33] grid_4.3.1          rstudioapi_0.15.0   lifecycle_1.0.3     nlme_3.1-162       
[37] vctrs_0.6.3         glue_1.6.2          farver_2.1.1        fansi_1.0.4        
[41] colorspace_2.1-0    purrr_1.0.1         tools_4.3.1         pkgconfig_2.0.3    

> nwk <- system.file("extdata", "sample.nwk", package="treeio")
> tree <- read.tree(nwk)
> p <- ggtree(tree) + geom_text2(aes(subset=!isTip, label=node), hjust=-.3) +
+   geom_tiplab()
> p
> MRCA(tree, tip=c('A', 'E'))
Error in MRCA.phylo(tree, tip = c("A", "E")) : 
  argument ".node1" is missing, with no default
> MRCA(p, tip=c('A', 'E'))
Error in MRCA.ggtree(p, tip = c("A", "E")) : 
  argument ".node1" is missing, with no default
> p$data[17,]
! # Invaild edge matrix for <phylo>. A <tbl_df> is returned.
# A tibble: 1 × 9
  parent  node branch.length label isTip     x     y branch angle
   <int> <int>         <dbl> <chr> <lgl> <dbl> <dbl>  <dbl> <dbl>
1     16    17            10 NA    FALSE    27  9.94     22  275.
> p + geom_hilight(node=18, fill="blue")
> p + geom_cladelab(node=18, label="Node 18")
Error in offspring.tbl_tree_item(.data = .data, .node = .node, tiponly = tiponly,  : 
  could not find function "offspring.tbl_tree_item"

geom_hilight() highlights only a very thin bad just at the internal node 17, and does not highlight the entire clade. p + geom_cladelab(node=18, label="Node 18") does not draw a cladogram.

I wonder if there is a conflict with some of my packages, but would like to avoid unloading and reloading hundreds of packages in my R framework.

I'm at a loss, and open to any suggestions. Thank you all for your time and attention!

ggtree • 4.6k views
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1
Entering edit mode

I started having this exact same problem with geom_hilight and geom_cladelab several days ago as well. I don't know why this is the case, but I was able to trace it to an update in the tidytree package. When I reverted back to a previous version of tidytree (see below), both functions started working again.

if(!require("tidytree", quietly=TRUE))
  install_version("tidytree", version = "0.4.2")
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Thanks! I just tried it and it is "working" again, and now I get this warning:

Warning messages: 1: The following aesthetics were dropped during statistical transformation: node, parent This can happen when ggplot fails to infer the correct grouping structure in the data. Did you forget to specify agroupaesthetic or to convert a numerical variable into a factor?

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0
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Hmm, I've not seen that warning before.

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@xshuangbin-21585
Last seen 12 months ago
Germany

Please refer to the answer.

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