Enter the body of text here I am new to R (R version 4.3.0) and RNAseq. As tutorial, I am using the paper 'RNA-Seq workflow: gene-level exploratory analysis and differential expression [version 2; peer review: 2 approved]" by Michael I Love et al and everything seems to well until now,
I have an error message stated below which worked well in the paper:
#Error in makeTxDbFromGFF(gtffile, format = "gtf", circ_seqs = character()) :
could not find function "makeTxDbFromGFF"
Please how do I solve it?
> bamfiles <- BamFileList(filenames, yieldSize = 2000000)
> seqinfo(bamfiles[1])
Seqinfo object with 84 sequences from an unspecified genome:
seqnames seqlengths isCircular genome
1 249250621 <NA> <NA>
10 135534747 <NA> <NA>
11 135006516 <NA> <NA>
12 133851895 <NA> <NA>
13 115169878 <NA> <NA>
... ... ... ...
GL000210.1 27682 <NA> <NA>
GL000231.1 27386 <NA> <NA>
GL000229.1 19913 <NA> <NA>
GL000226.1 15008 <NA> <NA>
GL000207.1 4262 <NA> <NA>
> gtffile <- file.path(dir,"Homo_sapiens.GRCh37.75_subset.gtf")
# > (txdb <- makeTxDbFromGFF(gtffile, format="gtf", circ_seqs=character()))
# Error in makeTxDbFromGFF(gtffile, format = "gtf", circ_seqs = character()) :
could not find function "makeTxDbFromGFF"