Recount3: PCR duplicates
1
0
Entering edit mode
@e4d785a9
Last seen 18 months ago
Netherlands

Hi,

I have been working with recount3 for quite some time now, I have read the documentation, but I cannot find any information on what is done with PCR duplicates. My question is then also if recount3 removed PCR duplicates (e.g. with picard or some other tool).

Kind regards, Seline

pcrduplicates recount3 multimappingreads • 972 views
ADD COMMENT
0
Entering edit mode

The maintainers might answer specifically for recount3, but in general PCR (or any) duplication is not removed in RNA-seq (or targeted assays in general) without presence of UMIs in the library prep as duplication (especially for highly-expressed genes) is expected and removal would unacceptably reduce the dynamic range of count. There is no reputable RNA-seq pipeline that to my knowledge does that.

ADD REPLY
0
Entering edit mode

The test tube containing the PCR mix will be placed in the incubation chamber of the Thermo cycler, which in Vietnam is often called a PCR machine. The temperature inside the incubation chamber of the baldi's basics PCR machine will change periodically, thanks to which DNA replication can take place

ADD REPLY
0
Entering edit mode
@lcolladotor
Last seen 1 day ago
United States

Hi,

You might be interested in this new README.md that Christopher Wilks made with information from Abhinav Nellore https://github.com/langmead-lab/monorail-external/blob/master/MULTIMAPPERS.md.

Best, Leonardo

ADD COMMENT
0
Entering edit mode

This addresses multimappers though, not PCR duplicates created during PCR amplification of the library in the lab.

ADD REPLY

Login before adding your answer.

Traffic: 867 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6