the data is Mature_miRNA_ID COV102 COV105 COV108 COV112 COV1 hsa-miR-548ap-3p 0 0 0 0 0 hsa-miR-548t-3p 0 0 0 0 0 hsa-miR-548e-3p 1 0 0 0 2 hsa-miR-548az-3p 0 0 0 0 0

for count data and metadata is id treatment COV102 test COV105 test COV108 test COV112 test COV1 test COV27 test COV33 test COV35 test

Code should be placed in three backticks as shown below

``` dds <- DESeqDataSetFromMatrix(countData=mycounts,colData=metadata,tidy=TRUE) converting counts to integer mode Error in DESeqDataSetFromMatrix(countData = mycounts, colData = metadata, : argument "design" is missing, with no default

``` how to solve this error

Please read the vignette to understand the very basics of how the software works. It will become clear then.

https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html

The design is the basis for DESeq2 to know what is shall compare and what to include into its model.