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Hello, I am using tximport to generate txi list from upstream kallisto output.
Then I use the txi list to construct a SummarizedExperiment object.
Here is the code for constructing the se object:
se <- SummarizedExperiment(assays = list(counts = txi$counts,
abundance = txi$abundance,
length = txi$length),
rowData = rownames(txi$counts),
colData = metadata)
So should I use the counts file for the downstream DESeq2 analysis?
> assays(se)
List of length 3
names(3): counts abundance length