Error in ImportRData
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Entering edit mode
@de366cac
Last seen 19 months ago
Brazil

I am trying to run some alternative splicing analysis for two biological triplicates using IsoformSwitchAnalyzeR (with a pipeline that worked before for me). But in the ImportRdata step the program keep returning this error in the import GTF step. I've searched in many forums but apparently nobody reported this error.

    <error/rlib_error_dots_nonempty>
Error in `dplyr::full_join()`:
! `...` must be empty.
✖ Problematic argument:
• all = TRUE
Run `rlang::last_trace()` to see where the error occurred.

and then 'rlang tracing' gives me this:

Backtrace:
     ▆
  1. └─IsoformSwitchAnalyzeR::importRdata(...)
  2.   ├─base::suppressWarnings(...)
  3.   │ └─base::withCallingHandlers(...)
  4.   └─IsoformSwitchAnalyzeR::importGTF(...)
  5.     ├─dplyr::full_join(...)
  6.     └─dplyr:::full_join.data.frame(...)
  7.       └─rlang::check_dots_empty0(...)
  8.         └─rlang::check_dots_empty(call = call)
  9.           └─rlang:::action_dots(...)
 10.             ├─base (local) try_dots(...)
 11.             └─rlang (local) action(...)

That is the commands I've used so far (the error stops in the importRdata part)

quants.df <- importIsoformExpression(parentDir=quants.dir, addIsofomIdAsColumn=TRUE)
design.df <- data.frame(sampleID=c("SRR1953775", "SRR1953776", "SRR1953777", "SRR1953778", "SRR1953779", "SRR19537710"), condition=c("Blood", "Blood", "Blood", "Lung", "Lung", "Lung"))

aSwitchlist <- importRdata(isoformCountMatrix=quants.df$counts, isoformRepExpression=quants.df$abundance, designMatrix=design.df, isoformExonAnnoation="/home/ramon/Documentos/PIBIC/eonSPE_altsplicing/coordinates_files/pteVam1.109.gtf", isoformNtFasta="/home/ramon/Documentos/PIBIC/eonSPE_altsplicing/coordinates_files/pteVam1.cdna.all.fa", fixStringTieAnnotationProblem=TRUE, showProgress=FALSE)
dplyr IsoformSwitchAnalyzeR • 1.8k views
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Entering edit mode

I have just started trying to get this workflow going and am having the same issue with my own data, and the supplied data within the package. Copy and pasting the code directly from https://www.bioconductor.org/packages/release/bioc/vignettes/IsoformSwitchAnalyzeR/inst/doc/IsoformSwitchAnalyzeR.html#analyzing-only-known-isoforms - also results in the same error.

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@035f6461
Last seen 18 months ago
Australia

I was able to fix this issue by updating IsoformSwitchAnalyzeR to the latest version on Github (v. 2.01.13)

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