Hi I just started using DEseq.I have one control sample and 12 different timepoints. I want to compare each timepoint to the control.Here is the sample meta file.

Sampl1  Control
Sampl2  D1
Sampl3  D12
Samp4   D14
Sampl5  D2
Sampl6  D21
Sampl7  D28
Sampl8  D3
Sampl9  D4
Sampl10 D5
Sampl11 D6
Sampl12 D7
Sampl13 D9

I want to compare Control vs D1, Control vs D12, Control vs D14.........control vs D9 etc I used the following code for one comparison

dds <- DESeqDataSetFromMatrix(countData=countData,colData=metaData,design=~Day, tidy = TRUE)
dds$Day<-factor(dds$Day, levels=c("Contro12","D12"))
dds<-DESeq(dds)

I get the following error
Error in designAndArgChecker(object, betaPrior) : 
  full model matrix is less than full rank
>

Can i get any help on this

DESeq2 requires at least one experimental group to be replicated, which is not the case. You cannot use it here. You can check the edgeR manual for "what to do without replicates" to do some exploratory analysis.