Dear all, I have a question about single cell RNA seq and ATAC seq. We want to conduct a single cell RNA seq for comparing the type of cell between group A, group B and group C ( A, B, C were the three types of the disease.). Also, we preforem the ATAC seq between the three groups.

How we could calculate the sample size for ATAC seq and single cell RNA seq, respectively?

Notably, we have found a paper titled "SCOPIT: sample size calculations for single-cell sequencing experiments" . But it provide a procedure for prospectively planning single-cell experiments or retrospectively evaluating if sufficient numbers of cells have been sequenced. (Not the number of patients）

How we could calculate the sample size for the number of patients for ATAC seq and single cell RNA seq, respectively? ( or We don't need to calculate it, we only need to calculated the number of cells)

Thank you for your help.