Question

What should I do with RNA-Seq data to get a gene that work?

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yuhang • 0

@1b2a94a4

Last seen 8 months ago

Hong Kong

Hi, everyone, I am a beginner with RNASeq and I got DEGs with Deseq2 base on the gene counts table. I have the enrichment results now, what should I do to find the genes that really work? Thanks!

find RNASeqRData • 850 views

ADD COMMENT • link updated 2.1 years ago by ATpoint ★ 4.5k • written 2.1 years ago by yuhang • 0

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Pleade define ‚work‘.

ADD REPLY • link 2.1 years ago ATpoint ★ 4.5k

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Thanks a lot for your reply! I want to find one or a few DEGs between the control and experimental groups and verify their function in vitro and in vivo. However, there are so many DEGs that I can not find the genes that lead to the differences between the control and experimental groups. I analyzed many KEGG pathways but could not connect those pathways together.

ADD REPLY • link 2.1 years ago yuhang • 0

score 1 · Answer 1 · 2022-10-26

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ATpoint ★ 4.5k

@atpoint-13662

Last seen 3 minutes ago

Germany

I think making sense of your data in the scientific context of your project is nothing that anyone here or in other communities can help you with.

ADD COMMENT • link 2.1 years ago ATpoint ★ 4.5k