Question

DESeq2 on normalized data

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lizaaa • 0

@8aed39be

Last seen 2.4 years ago

Russia

I've recently started using DESeq2. Unfortunately, I don't have raw counts data but have normalized counts. How can I analyse gene expression for already normalized counts using DESeq2?

DESeq2 • 1.4k views

ADD COMMENT • link 2.5 years ago lizaaa • 0

score 0 · Answer 1 · 2022-08-18

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ATpoint ★ 4.6k

@atpoint-13662

Last seen 2 hours ago

Germany

It’s not supported. Use limma-trend with the log2-transformed normalized counts if that is all you have. See limma manual.

ADD COMMENT • link 2.5 years ago ATpoint ★ 4.6k

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Thank you!

ADD REPLY • link 2.5 years ago lizaaa • 0