Can I force flowStats::quadrantGate to pick a separation point on the upper side of a signal distribution?
0
0
Entering edit mode
@5bc47b34
Last seen 2.3 years ago
United States

I'm trying to use a quad gate to identify CD56++ (double positive) CD16+ cells. As such, I'm trying to force the quadrantGate function do find the separation point on the high side of the major CD56 signal peak (the CD16 separation point is perfect). Unfortunately I can only get it to identify the separation point on the low side for CD56 which is not what what I want. Grateful for any suggestions.

flowStats::quadrantGate(df, stains=c("CD16", "CD56"), plot=TRUE)

Current quadrantGate results

flowStats quadrantGate • 692 views
ADD COMMENT
0
Entering edit mode

To answer my own question, using the rare=TRUE option moves the CD56 gate to the top of the distribution quite nicely.

ADD REPLY

Login before adding your answer.

Traffic: 1005 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6