Can I force flowStats::quadrantGate to pick a separation point on the upper side of a signal distribution?
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@5bc47b34
Last seen 2.3 years ago
United States

I'm trying to use a quad gate to identify CD56++ (double positive) CD16+ cells. As such, I'm trying to force the quadrantGate function do find the separation point on the high side of the major CD56 signal peak (the CD16 separation point is perfect). Unfortunately I can only get it to identify the separation point on the low side for CD56 which is not what what I want. Grateful for any suggestions.

flowStats::quadrantGate(df, stains=c("CD16", "CD56"), plot=TRUE)

Current quadrantGate results

flowStats quadrantGate • 700 views
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To answer my own question, using the rare=TRUE option moves the CD56 gate to the top of the distribution quite nicely.

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