Hi,

I am using ChIPseeker_1.28.3 for my peak data annotation. I appreciate that I can use this great tool.

I have a question about 'Distal Intergenic.' I read your paper and saw GitHub pages, but I can't find it.

After running 'annotatePeak' function, the output is peak annotations, such as Promoter (<=1kb), Promoter (1-2kb) ... Downstream (<=300), and Distal Intergenic.

peakAnno_Output <- annotatePeak(MyPeakBedFile, tssRegion=c(-3000, 3000), TxDb=txdb, annoDb="org.Hs.eg.db")

As we know, 'Intergenic' is DNAs located between genes. This 'intergenic' region doesn't include Promoter, 5' UTR, and 3' UTR. Then, could you tell me how you defined "Distal Intergenic"? It sounds like it excludes 5' UTR and 3' UTR, so it is named as 'distal intergenic.' Could you tell how you defined 'distal intergenic'? Do you have specific sequence length that should be apart from 5' UTR and 3'UTR in order to define distal intergenic?

Thank you, Sanghoon