Hello all

I am trying to analyze RNA-seq data at 3 different time points. I have two replicates for two time points while 1 replicate at the 0 timepoint. All the samples are with the same condition and I want to know the differentially expressed genes at data0h vs data6h vs data12h. As I am new to this kind of analysis, I am wondering if a ANOVA like analysis solve the purpose or if I should consider the glmQLFTest from EdgeR.

```

I would just compare each timepoint to baseline using the quasi-likelihood pipeline (which includes glmQLFit and glmQLFTest).

glmQLFTest can do F-tests just like anova, so it can achieve all the same purposes as an anova approach.