Hi Julie, Kai,

As I am looking at the offtargets output I was wondering if I could get read counts too? Or, if I missed it somewhere?

Just FYI - these arguments must be obsolete... Error in GUIDEseqAnalysis(alignment.inputfile, umi.inputfile, BSgenomeName = Hsapiens, : unused arguments (min.umi.count = 5, max.umi.count = 100000, min.read.coverage = 1)

Thanks Sharvari

# include your problematic code here with any corresponding output 
# please also include the results of running the following in an R session 

sessionInfo( )