Hello everyone, I use DEseq2 and Im working on R studio, I used the below commands for running the DEseq2:

library(DESeq2)
counts_data <- read.csv("/Users/maryam/Documents/comp1/allcountsss.csv") colData <- read.table("/Users/maryam/Documents/comp1/Book1.csv",header = TRUE,sep = ",")
head(colData)

colData2 <- cbind(colData,as.factor(colData$condition))[,-2]
cbind(colData,as.factor(colData$condition))[,-2]
summary(colData2)

dds <- DESeqDataSetFromMatrix(countData = counts_data[,2:5], colData = colData2, design = ~ condition)

but I am getting this error:
Error in DESeqDataSet(se, design = design, ignoreRank) :
all variables in design formula must be columns in colData

I checked my design formula it is like this:
ensgeneID as.factor(colData$condition)

1. ESN0000.... control

   1. ENS0000... control
   2. ENS0000.... mix1
2. ENS000..... mix2

I looked for solution on google but exactly can't find the problem, is there anyone to help me please?

Your colData2 object must contain a column condition