Illumina 450k Manifest: Probes Mapping to Multiple RefGene Groups and Names
2
0
Entering edit mode
metamaden ▴ 10
@madensean-8348
Last seen 12 months ago
United States

From the 450k manifest data that I retrieve using getAnnotation() in minfi, how do I interpret variables UCSC_RefGene_Name and UCSC_RefGene_Group when there are several genes or groups listed (separated by a semicolon) for the same probe (that is, in the same row)?

Example:

For probe X, I have:

UCSC_RefGene_Name: Gene1;Gene2;Gene1

UCSC_RefGene_Group: TSS200;Body;1stExon

My guess for this probe would be that it maps to three distinct transcripts: 1. In TSS200 of first Gene1 transcript; 2. In Body of Gene2 transcript; 3. in 1stExon of second Gene1 transcript. Is this correct?

Best,

Sean

 

 

 

illuminahumanmethylation450kanno.ilmn12.hg19 minfi illuminahumanmethylation450k.db methylation • 3.1k views
ADD COMMENT
0
Entering edit mode
@kasper-daniel-hansen-2979
Last seen 17 months ago
United States
This annotation is provided by Illumina and is unchanged. I don't use it myself, and have no comments on it. Best, Kasper On Fri, Oct 2, 2015 at 4:31 PM, maden.sean [bioc] <noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User maden.sean <https: support.bioconductor.org="" u="" 8348=""/> wrote Question: > Illumina 450k Manifest: Probes Mapping to Multiple RefGene Groups and Names > <https: support.bioconductor.org="" p="" 72915=""/>: > > From the 450k manifest data that I retrieve using getAnnotation() in > minfi, how do I interpret variables UCSC_RefGene_Name and > UCSC_RefGene_Group when there are several genes or groups listed (separated > by a semicolon) for the same probe (that is, in the same row)? > > Example: > > For probe X, I have: > > UCSC_RefGene_Name: Gene1;Gene2;Gene1 > > UCSC_RefGene_Group: TSS200;Body;1stExon > > My guess for this probe would be that it maps to three distinct > transcripts: 1. In TSS200 of first Gene1 transcript; 2. In Body of Gene2 > transcript; 3. in 1stExon of second Gene1 transcript. Is this correct? > > Best, > > Sean > > > > > > > > ------------------------------ > > Post tags: illuminahumanmethylation450kanno.ilmn12.hg19, minfi, > illuminahumanmethylation450k.db, methylation > > You may reply via email or visit Illumina 450k Manifest: Probes Mapping to Multiple RefGene Groups and Names >
ADD COMMENT
0
Entering edit mode
Will • 0
@12a42d52
Last seen 2.6 years ago
Canada

The manifest has a description of each entry available here. It states that different spliceforms are listed, and the Groups are listed in that same order. I would say that your interpretation is correct

Best, Will

ADD COMMENT

Login before adding your answer.

Traffic: 827 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6