Enter the body of text here

Code should be placed in three backticks as shown below

Hi! i am trying DESeq2 analysis but facing an issue when I used this command, can anyone help me, please.

dds <- DESeqDataSetFromMatrix(count_matrix = count_matrix, col_data = col_data, design = ~ condition) dds <- DESeqDataSetFromMatrix(count_matrix = count_matrix,

• col_data = col_data,
• design = ~ condition) Error in h(simpleError(msg, call)) : error in evaluating the argument 'x' in selecting a method for function 'ncol': argument "countData" is missing, with no default

  include your problematic code here with any corresponding output

  please also include the results of running the following in an R session

sessionInfo( )

Use ?DESeqDataSetFromMatrix, the argument in the function to accept the counts is countData, not count_matrix. It's also colData and not col_data.

dds <- DESeqDataSetFromMatrix(countData= count_matrix, colData= col_data, design = ~ condition)