Entering edit mode
Hi everyone,
I have two HiC data from two different conditions. I would like to calculate the differential interaction between these two conditions using HiCDCplus
package. Is it possible to run hicdiff
without replicates?
if yes, how?
if no, is there any other way to do so from .hic files?
Thanks much
Best,
TA
hicdcdiff
relies onDESeq2
, which doesn't allow comparison of two conditions without replicates as significances cannot be determined reliably. You can check https://www.biostars.org/p/398152/ for a comprehensive set of discussions and links relating to this matter.