Glimma: Error: Length of groups must be equal to the number of columns in counts
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Jonas • 0
@f91ff31c
Last seen 2.1 years ago
United Kingdom

Hi, I was having an issue when using Glimma package to generate plots. My data processing before Glimma was in DESeq2.

dds = DESeq(dds)
glimmaMA(dds)

I tried another plot glimmaMDS(dds), it was operating normally. However, whenever I try the glimmaMA or glimmaVolcano it doesnt work, and the error message is the same:

302 of 12813 genes were filtered out in DESeq2 tests Error in buildXYData(table, status, main, display.columns, anno, counts, : Length of groups must be equal to the number of columns in counts.

The R feedback was

  1. stop("Length of groups must be equal to the number of columns in counts.\n")
  2. buildXYData(table, status, main, display.columns, anno, counts, xlab, ylab, status.cols, sample.cols, groups, transform.counts)
  3. glimmaVolcano.DESeqDataSet(dds)
  4. glimmaVolcano(dds)

Could you please help with potential cause and solutions regarding this error? Thank you very much!!

Glimma RNASeq • 1.3k views
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