Hi I'm using a recent version of Enhancedvolcano and using data of 3 months ago where I got volcano plots with labels next to the points, today I get labels that are right on top of the points. Is there a way to nudge these left or right top or bottom? here's my code
plot1 <- EnhancedVolcano(mydata,
lab = as.character(mydata$gene),
x = "log2_fold_change",
y = "adj_p",
title = "Edu+ vs EdU- Microglia",
xlim = c(-8,10),
ylim = c(0,8),
selectLab = c(),
pCutoff = 0.05,
FCcutoff = 0.5849,
labSize = 5.0,
pointSize = 3,
col=c('grey10', 'royalblue4', 'purple4', 'red3'),
colAlpha = 0.3,
cutoffLineType = 'dashed',
cutoffLineCol = 'black',
cutoffLineWidth = 0.4,
legendLabels=c('Not sig.','Log2 FC','p-value',
'p-value & Log2FC'),
legendPosition = 'right',
legendLabSize = 10,
legendIconSize = 3.0,
gridlines.major = FALSE,
gridlines.minor = FALSE,
#drawConnectors = TRUE,
#widthConnectors = 0.75
)
plot1
Ok thx Kevin, I’ll use connectors and make them white or something do I don’t see them. Alternatively I can just nudge them in illustrator. Thx for your time
Sure thing, okay, it is difficult to account for every situation. Best of luck