DiffBind (Error in if (DBA$config$parallelPackage))
1
0
Entering edit mode
eggrandio • 0
@eggrandio-14403
Last seen 2.5 years ago
United States

Hi,

I am trying to use the DiffBind package but I get the following error:

wtvspq.count = dba.count(wtvspq[1:2], bParallel = FALSE)
Computing summits...
Error in if (DBA$config$parallelPackage == DBA_PARALLEL_MULTICORE) { : 
  argument is of length zero

I am able to create a dba object, and even changing the config to RunParallel=FALSE, I get the same error. I had problems in the past using DiffBind on Windows, but at least I was able to run it serially.

wtvspq = dba(sampleSheet="reduced.csv", dir="C:/DATA/BAM_as_BED/",
             config=data.frame(AnalysisMethod=DBA_DESEQ2,th=0.05,
                      DataType=DBA_DATA_GRANGES, RunParallel=FALSE, 
                      minQCth=15, fragmentSize=125, 
                      bCorPlot=FALSE, reportInit="DBA", 
                      bUsePval=FALSE, design=TRUE,
                      doBlacklist=TRUE, doGreylist=TRUE))

Here is my sessionInfo in case it helps:

sessionInfo()
R version 4.1.1 (2021-08-10)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 19043)

Matrix products: default

locale:
[1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252    LC_MONETARY=English_United States.1252
[4] LC_NUMERIC=C                           LC_TIME=English_United States.1252    

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] DiffBind_3.2.5              SummarizedExperiment_1.22.0 Biobase_2.52.0              MatrixGenerics_1.4.2       
 [5] matrixStats_0.60.0          GenomicRanges_1.44.0        GenomeInfoDb_1.28.1         IRanges_2.26.0             
 [9] S4Vectors_0.30.0            BiocGenerics_0.38.0        

loaded via a namespace (and not attached):
  [1] backports_1.2.1          GOstats_2.58.0           BiocFileCache_2.0.0      plyr_1.8.6               GSEABase_1.54.0         
  [6] splines_4.1.1            BiocParallel_1.26.1      ggplot2_3.3.5            amap_0.8-18              digest_0.6.27           
 [11] invgamma_1.1             htmltools_0.5.1.1        viridis_0.6.1            GO.db_3.13.0             SQUAREM_2021.1          
 [16] fansi_0.5.0              magrittr_2.0.1           checkmate_2.0.0          memoise_2.0.0            BSgenome_1.60.0         
 [21] base64url_1.4            limma_3.48.3             Biostrings_2.60.1        annotate_1.70.0          systemPipeR_1.26.3      
 [26] bdsmatrix_1.3-4          prettyunits_1.1.1        jpeg_0.1-9               colorspace_2.0-2         blob_1.2.2              
 [31] rappdirs_0.3.3           apeglm_1.14.0            ggrepel_0.9.1            xfun_0.24                dplyr_1.0.7             
 [36] crayon_1.4.1             RCurl_1.98-1.3           jsonlite_1.7.2           graph_1.70.0             genefilter_1.74.0       
 [41] brew_1.0-6               survival_3.2-12          VariantAnnotation_1.38.0 glue_1.4.2               gtable_0.3.0            
 [46] zlibbioc_1.38.0          XVector_0.32.0           DelayedArray_0.18.0      V8_3.4.2                 Rgraphviz_2.36.0        
 [51] scales_1.1.1             mvtnorm_1.1-2            pheatmap_1.0.12          DBI_1.1.1                edgeR_3.34.0            
 [56] Rcpp_1.0.7               viridisLite_0.4.0        xtable_1.8-4             progress_1.2.2           emdbook_1.3.12          
 [61] bit_4.0.4                rsvg_2.1.2               truncnorm_1.0-8          AnnotationForge_1.34.0   httr_1.4.2              
 [66] gplots_3.1.1             RColorBrewer_1.1-2       ellipsis_0.3.2           pkgconfig_2.0.3          XML_3.99-0.6            
 [71] dbplyr_2.1.1             locfit_1.5-9.4           utf8_1.2.1               tidyselect_1.1.1         rlang_0.4.11            
 [76] AnnotationDbi_1.54.1     munsell_0.5.0            tools_4.1.1              cachem_1.0.5             generics_0.1.0          
 [81] RSQLite_2.2.7            broom_0.7.9              evaluate_0.14            stringr_1.4.0            fastmap_1.1.0           
 [86] yaml_2.2.1               knitr_1.33               bit64_4.0.5              caTools_1.18.2           purrr_0.3.4             
 [91] KEGGREST_1.32.0          dendextend_1.15.1        RBGL_1.68.0              xml2_1.3.2               biomaRt_2.48.3          
 [96] compiler_4.1.1           rstudioapi_0.13          filelock_1.0.2           curl_4.3.2               png_0.1-7               
[101] tibble_3.1.2             stringi_1.7.3            GenomicFeatures_1.44.1   lattice_0.20-44          Matrix_1.3-4            
[106] vctrs_0.3.8              pillar_1.6.2             lifecycle_1.0.0          irlba_2.3.3              data.table_1.14.0       
[111] bitops_1.0-7             rtracklayer_1.52.1       R6_2.5.1                 BiocIO_1.2.0             latticeExtra_0.6-29     
[116] hwriter_1.3.2            ShortRead_1.50.0         KernSmooth_2.23-20       gridExtra_2.3            MASS_7.3-54             
[121] gtools_3.9.2             assertthat_0.2.1         Category_2.58.0          rjson_0.2.20             withr_2.4.2             
[126] GenomicAlignments_1.28.0 batchtools_0.9.15        Rsamtools_2.8.0          GenomeInfoDbData_1.2.6   hms_1.1.0               
[131] grid_4.1.1               coda_0.19-4              tidyr_1.1.3              DOT_0.1                  rmarkdown_2.10          
[136] GreyListChIP_1.24.0      ashr_2.2-47              mixsqp_0.3-43            bbmle_1.0.24             numDeriv_2016.8-1.1     
[141] tinytex_0.33             restfulr_0.0.13
parallel R DiffBind • 1.7k views
ADD COMMENT
3
Entering edit mode
Rory Stark ★ 5.2k
@rory-stark-5741
Last seen 27 days ago
Cambridge, UK

I am checking in a fix for this case where a config record is specified without a value for parallelPackage. It will then run in serial mode from version 3.2.6 onwards.

You can workaround this by adding a value for config$parallelPackage to your config data.frame. For example, add parallelPackage=0 to aways run serially, or parallelPackage=1 to enable parallel execution, in your call to dba() where you specify the config values.

ADD COMMENT
0
Entering edit mode

That sounds good. Although I do not know why, I was getting that error message also without specifying a config file. That's why I tried RunParallel=FALSE, but I guess parallelPackage still needs a value.

ADD REPLY
0
Entering edit mode

Hi Rory,

Thank you for clarifying and for the awesome R package! I was just wondering if there is any update on this as I seem to still be experiencing this issue in 3.4.0.

ADD REPLY
0
Entering edit mode

I added some more checks in DiffBind_3.4.2 to make sure it runs correctly on Windows.

ADD REPLY

Login before adding your answer.

Traffic: 630 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6