HiC-DC+ Distance / Model Fitting error when running HiCDCPlus()
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Entering edit mode
Hannah • 0
@02a93254
Last seen 3.2 years ago
United States

Hi, I am working with some HiChIP data, starting from HiC-Pro outputs. I am running into an error when running HiCDCPlus(gi_list) - see below. I have tried loading the HiC data via the allValidPairs file as well as using the matrix + bed file combination.

Thanks in advance for any help!


# include your problematic code here with any corresponding output 

gi_list_hicpro <- add_hicpro_matrix_counts(
+        gi_list,
+        paste0(hic_mat_dir,"DMSO_rep1_chr_500000_abs.bed"),
+        paste0(hic_mat_dir,"DMSO_rep1_500000.matrix"),
+        add_inter = FALSE)

> gi_list_expand1D<-expand_1D_features(gi_list_hicpro)
> set.seed(1010) #HiC-DC downsamples rows for modeling
> gi_list_hicdcplus<-HiCDCPlus(gi_list_expand1D) #HiCDCPlus_parallel runs in parallel across ncores
Chromosome chr1 complete.
Chromosome chr10 complete.
Error in splineDesign(Aknots, x, ord) : 
  length of 'derivs' is larger than length of 'x'
> gi_list_hicdcplus<-HiCDCPlus(gi_list_expand1D, distance_type="log") #HiCDCPlus_parallel runs in parallel across ncores
Chromosome chr1 complete.
Chromosome chr10 complete.
Error in glm.fit(x = numeric(0), y = numeric(0), weights = NULL, start = NULL,  : 
  object 'fit' not found

# please also include the results of running the following in an R session 
> sessionInfo()
R version 4.0.1 (2020-06-06)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: CentOS Linux 7 (Core)

Matrix products: default
BLAS/LAPACK: /n/app/openblas/0.2.19/lib/libopenblas_core2p-r0.2.19.so

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets 
[8] methods   base     

other attached packages:
 [1] BSgenome.Hsapiens.UCSC.hg19_1.4.3 BSgenome_1.58.0                  
 [3] rtracklayer_1.50.0                Biostrings_2.58.0                
 [5] XVector_0.30.0                    GenomicRanges_1.42.0             
 [7] GenomeInfoDb_1.26.7               IRanges_2.24.1                   
 [9] S4Vectors_0.28.1                  BiocGenerics_0.36.1              
[11] HiCDCPlus_0.99.14                

loaded via a namespace (and not attached):
  [1] ProtGenerics_1.22.0         bitops_1.0-7               
  [3] matrixStats_0.60.0          bit64_4.0.5                
  [5] RColorBrewer_1.1-2          progress_1.2.2             
  [7] httr_1.4.2                  InteractionSet_1.18.1      
  [9] backports_1.2.1             tools_4.0.1                
 [11] utf8_1.2.2                  R6_2.5.0                   
 [13] rpart_4.1-15                lazyeval_0.2.2             
 [15] Hmisc_4.5-0                 DBI_1.1.1                  
 [17] Gviz_1.34.1                 colorspace_2.0-2           
 [19] nnet_7.3-16                 gridExtra_2.3              
 [21] tidyselect_1.1.1            prettyunits_1.1.1          
 [23] bit_4.0.4                   curl_4.3.2                 
 [25] compiler_4.0.1              Biobase_2.50.0             
 [27] htmlTable_2.2.1             xml2_1.3.2                 
 [29] DelayedArray_0.16.3         checkmate_2.0.0            
 [31] scales_1.1.1                askpass_1.1                
 [33] rappdirs_0.3.3              stringr_1.4.0              
 [35] digest_0.6.27               Rsamtools_2.6.0            
 [37] foreign_0.8-80              R.utils_2.10.1             
 [39] dichromat_2.0-0             htmltools_0.5.1.1          
 [41] base64enc_0.1-3             jpeg_0.1-9                 
 [43] pkgconfig_2.0.3             MatrixGenerics_1.2.1       
 [45] ensembldb_2.14.1            dbplyr_2.1.1               
 [47] fastmap_1.1.0               htmlwidgets_1.5.3          
 [49] rlang_0.4.11                rstudioapi_0.13            
 [51] RSQLite_2.2.7               generics_0.1.0             
 [53] BiocParallel_1.24.1         dplyr_1.0.7                
 [55] R.oo_1.24.0                 VariantAnnotation_1.34.0   
 [57] RCurl_1.98-1.3              magrittr_2.0.1             
 [59] GenomeInfoDbData_1.2.4      Formula_1.2-4              
 [61] Matrix_1.3-4                Rcpp_1.0.7                 
 [63] munsell_0.5.0               fansi_0.5.0                
 [65] lifecycle_1.0.0             R.methodsS3_1.8.1          
 [67] stringi_1.6.2               MASS_7.3-54                
 [69] SummarizedExperiment_1.20.0 zlibbioc_1.36.0            
 [71] BiocFileCache_1.14.0        grid_4.0.1                 
 [73] blob_1.2.2                  crayon_1.4.1               
 [75] lattice_0.20-41             splines_4.0.1              
 [77] GenomicFeatures_1.42.3      hms_1.1.0                  
 [79] knitr_1.33                  pillar_1.6.2               
 [81] igraph_1.2.6                biomaRt_2.46.3             
 [83] XML_3.99-0.6                glue_1.4.2                 
 [85] biovizBase_1.38.0           latticeExtra_0.6-29        
 [87] data.table_1.14.0           vctrs_0.3.8                
 [89] png_0.1-7                   gtable_0.3.0               
 [91] openssl_1.4.4               purrr_0.3.4                
 [93] tidyr_1.1.3                 assertthat_0.2.1           
 [95] cachem_1.0.5                ggplot2_3.3.5              
 [97] xfun_0.24                   AnnotationFilter_1.14.0    
 [99] survival_3.2-11             tibble_3.1.2               
[101] GenomicAlignments_1.26.0    AnnotationDbi_1.52.0       
[103] memoise_2.0.0               cluster_2.1.2              
[105] ellipsis_0.3.2              GenomicInteractions_1.24.0
HiC-DC+ HiCDCPlus • 774 views
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Entering edit mode

I think the df and splineknotting options are yielding distance spline points that are outside the distance range available in the data. I would recommend trying lower df and/or splineknotting="count-based"

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