Hi,

I have read the vignette on the Bioconductor package TCSeq for time series RNA seq analysis and now I'm trying to implement it on my dataset. To create a TCA object I used my counts matrix, where each column is a different timepoint and the rows correspond to the ensembl gene ID.

What I can't figure out is the genomicFeature variable of the TCA function, which requires the genomic intervals. Since in the counts matrix I have, already has the corresponding genes/ensembl gene ID, I would probably need a matrix with the chromosomal annotation for each genes/ensembl gene ID, no? Or how would you solve this conundrum?

Thank you!