WGCNA Co-expression
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munirapcu • 0
@munirapcu-12086
Last seen 7.7 years ago

Hello Everyone,

 

              How to use differential expressed genes (DEG) as input data for module detection ?. Because, I am unable to understand proper codes for this experiment.

 

 
microarray differential gene expression module • 1.6k views
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@peter-langfelder-4469
Last seen 4 weeks ago
United States

Please read WGCNA FAQ, item 2, at

https://labs.genetics.ucla.edu/horvath/CoexpressionNetwork/Rpackages/WGCNA/faq.html

We don't recommend filtering genes by differential expression.

As for using WGCNA, there are several tutorials at https://labs.genetics.ucla.edu/horvath/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/index.html. They will show you how to use the package.
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these papers (Identification of hub genes of pneumocyte senescence induced by thoracic irradiation using weighted gene co‑expression network analysis, Integration of Gene Coexpression Network, GO Enrichment Analysis for
Identification Gene Expression Signature of Invasive Bladder Carcinoma)  and like other papers they have clearly mentioned that, they have taken DEGs for module detection in WGCNA. 

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@peter-langfelder-4469
Last seen 4 weeks ago
United States

Some people do it despite (or not knowing) our recommendation, perhaps they have good reasons to do so. Still, the general recommendation is not to filter genes by DE.

I suggest you work through the tutorials and run your own analysis along the lines of the tutorial.
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