Dear all, On running the eBayes function in LIMMA, I got the following error. > fit_NODS7 <- eBayes(fit_NODS7) Warning message: Estimation of var.prior failed - set to default value in: ebayes(fit = fit, proportion = proportion, stdev.coef.lim = stdev.coef.lim) What does it mean? I know that the data is passed from the lmFit() step to the eBayes() step without intervention from the user. It worked for a couple of experiments and for the third set, gave the above error. Thank you, -Ankit

Dear all, Sorry to repeat the question, but since I received no reply for the same, I had to. On running the eBayes function in LIMMA, I got the following error. > fit_NODS7 <- eBayes(fit_NODS7) Warning message: Estimation of var.prior failed - set to default value in: ebayes(fit = fit, proportion = proportion, stdev.coef.lim = stdev.coef.lim) What does it mean? I know that the data is passed from the lmFit() step to the eBayes() step without intervention from the user. It worked for a couple of experiments and for the third set, gave the above error. Thank you, -Ankit