I have just learned how to use DESeq2 and had written a tutorial for my bioinformatics class.  Unfortunately, I made the mistake of updating R to version 3.3.2 (running Mac OS X 10.11) and it seems that DESeq2 is no longer supported.  This is hugely disappointing.  It ran on R-3.3.0 (may 2016), but I’m having trouble getting this archived version back from CRAN.

Any suggestions would be appreciated.

Here's the error message from R console:

install.packages("DESeq2")
Warning in install.packages :
  package ‘DESeq2’ is not available (for R version 3.3.2)

The mistake is using install.packages() rather than biocLite() to do the installation. From the DESeq2 Bioconductor page:

Installation

To install this package, start R and enter:

## try http:// if https:// URLs are not supported
source("https://bioconductor.org/biocLite.R")
biocLite("DESeq2")

Please try to install the package following the Bioconductor installation instructions preset on every package landing page

source("https://bioconductor.org/biocLIite.R")
biocLite("DESeq2")

The package is very much available and actively supported.

Be sure to validate your current installation, since if you have been using install.packages() directly it is likely to be invalid

BiocInstaller::biocValid()