DESeq2 error when creating the DESeqDataSet
1
0
Entering edit mode
@sebastianlobentanzer-11790
Last seen 3.5 years ago
Germany

I am trying to create a DESeqDataSet from a SummarizedExperiment. I am getting the error message:

Error in (function (classes, fdef, mtable) : unable to find an inherited method for function ‘assayNames’ for signature ‘"DESeqDataSet"’

While researching, I found this (verified) example to test with:

df=data.frame("treat"=sample(c(80:100),6, replace=FALSE),
              "treat1"=sample(c(90:103),6, replace=FALSE),
              "treat2"=sample(c(80:100),6, replace=FALSE),
              "ctrl"=sample(c(60:90),6, replace=FALSE),
              "ctrl1"=sample(c(60:90),6, replace=FALSE),
              "ctrl2"=sample(c(60:90),6, replace=FALSE))
conds<-as.factor(c("Treat","Treat","Treat","Control","Control","Control"))
coldata <- data.frame(row.names=colnames(df), conds)
dds=DESeqDataSetFromMatrix(countData=df,colData=coldata,design=~conds)

Unfortunately, this example gives the same error. I tried the "airway" example, same thing happenend. My sessionInfo() is:

R version 3.3.1 (2016-06-21) Platform: x86_64-apple-darwin13.4.0 (64-bit) Running under: OS X 10.11.6 (El Capitan)

locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages: [1] stats4 parallel stats graphics grDevices utils datasets methods base

other attached packages: [1] DESeq2_1.10.1
RcppArmadillo_0.7.500.0.0 Rcpp_0.12.7
SummarizedExperiment_1.4.0 Biobase_2.34.0
GenomicRanges_1.26.1 [7] GenomeInfoDb_1.10.0
IRanges_2.8.0 S4Vectors_0.12.0
BiocInstaller_1.20.3 AnnotationHub_2.2.5
BiocGenerics_0.20.0

loaded via a namespace (and not attached): [1] locfit_1.5-9.1
lattice_0.20-34 Rsamtools_1.26.1
Biostrings_2.42.0 digest_0.6.10 [6] mime_0.5 R6_2.2.0 plyr_1.8.4
chron_2.3-47 acepack_1.4.1 [11] ShortRead_1.28.0 RSQLite_1.0.0 httr_1.2.1
ggplot2_2.1.0 zlibbioc_1.20.0 [16] GenomicFeatures_1.26.0 data.table_1.9.6
annotate_1.48.0 rpart_4.1-10
Matrix_1.2-7.1 [21] splines_3.3.1
BiocParallel_1.8.0 geneplotter_1.48.0
stringr_1.1.0 foreign_0.8-67 [26] RCurl_1.95-4.8 biomaRt_2.30.0
munsell_0.4.3 shiny_0.14.2 httpuv_1.3.3 [31] rtracklayer_1.34.0 htmltools_0.3.5
nnet_7.3-12 gridExtra_2.2.1
htmlTable_1.7 [36] interactiveDisplayBase_1.8.0 Hmisc_4.0-0 XML_3.98-1.4
GenomicAlignments_1.10.0 bitops_1.0-6 [41] grid_3.3.1 xtable_1.8-2 gtable_0.2.0 DBI_0.5-1 magrittr_1.5 [46] scales_0.4.0 stringi_1.1.2
XVector_0.14.0 hwriter_1.3.2
genefilter_1.52.1 [51] latticeExtra_0.6-28
Formula_1.2-1 RColorBrewer_1.1-2 tools_3.3.1 survival_2.40-1 [56] AnnotationDbi_1.36.0
colorspace_1.2-7 cluster_2.0.5 knitr_1.14

traceback():

11: stop(gettextf("unable to find an inherited method for function %s for signature %s", sQuote(fdef@generic), sQuote(cnames)), domain = NA) 
10: (function (classes, fdef, mtable) { methods <- .findInheritedMethods(classes, fdef, mtable) if (length(methods) == 1L) return(methods[[1L]]) else if (length(methods) == 0L) { cnames <- paste0("\"", vapply(classes, as.character, ""), "\"", collapse = ", ") stop(gettextf("unable to find an inherited method for function %s for signature %s", sQuote(fdef@generic), sQuote(cnames)), domain = NA) } else stop("Internal error in finding inherited methods; didn't return a unique method", domain = NA) })(list("DESeqDataSet"), function (x, ...) standardGeneric("assayNames"), ) 
9: assayNames(object) 
8: "counts" %in% assayNames(object) 
7: validityMethod(object) 
6: anyStrings(validityMethod(object)) 
5: validObject(.Object) 
4: initialize(value, ...) 
3: initialize(value, ...) 
2: new("DESeqDataSet", se, design = design) 
1: DESeqDataSet(se, design = ~cell + dex)
deseq2 bioconductor deseqdataset r summarizedexperiment • 4.4k views
ADD COMMENT
2
Entering edit mode
@sebastianlobentanzer-11790
Last seen 3.5 years ago
Germany

Solution: I recompiled all Bioconductor packages with

source("https://bioconductor.org/biocLite.R")
pkgs <- rownames(installed.packages())
biocLite(pkgs, type="source")

and then restarted.

ADD COMMENT

Login before adding your answer.

Traffic: 617 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6