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Yashwant
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10
@yashwant-6506
Last seen 8.0 years ago
I have three biological replicates of a tissue and each biological
replicates
has three technical replicates. There is little difference between
biological
replicates however technical replicates are almost same. I would like
to use
quantile normalization method between biological replicates.
matrix at present is as follow:
Biological replicate 1 Biological
replicate2 Biological replicate 3
Tech. rep 1a 1b 1c Tech.rep
2a 2b 2c
Tech. rep 3a 3b 3c
Gene1 1.23 1.24 1.57 2.43
2.37
2.40 1.97 1.85 1.75
Gene2 7.85 6.23 7.50 5.55
5.45
5.67 8.57 8.32 8.52
I have to normalize this data taking consideration of inter biological
replicate quantile normalization.