Assesment of the number of signatures when using SomaticSignatures package
1
1
Entering edit mode
arsala521 ▴ 10
@arsala521-10976
Last seen 8.4 years ago

Hello

I am analyzing data through SomaticSignatures package. I am trying to use assesNumberSignatures function to determine number of signatures.

I entered the following commands:

> n_sigs = 2:8
> gof_pca = assessNumberSignatures(cancer_mm, n_sigs, pcaDecomposition​)

When I entered the second command, I got the following error:

Error in findSignatures(m, nSigs, decomposition, ...)

'nSigs|r' must be a single positive integer in the range [1,4]

Would someone please help me in this?

Regards

 

assesNumberSignatures SomaticSignatures package • 1.4k views
ADD COMMENT
1
Entering edit mode
@wolfgang-huber-3550
Last seen 3 months ago
EMBL European Molecular Biology Laborat…

Have a look at the error message and perhaps you will note from it that 8 is larger than 4. Therefore, try with n_sigs=2:4.

ADD COMMENT
0
Entering edit mode

I did try with 2:4. I got the plot but I was bit confused in choosing the number of signatures from the given plot.

As number of signatures has to be chosen such that with increasing the number of signatures the RSS and the explained variance do not change sufficiently. When I plotted using n_sigs=2:4, the resulting plot showed that RSS and explained variance changed till number of signatures = 4. I was not able to judge from the graph that whether with increasing the number of signatures beyond 4, the RSS and explained variance would change or not.

How would I choose number of signatures in such case?

ADD REPLY

Login before adding your answer.

Traffic: 623 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6