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Xiao Shi
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90
@xiao-shi-1184
Last seen 10.2 years ago
hi,everybody
I have a *time course* data set about a CML cell line treated by two
drugs
and their combination.The experiment was performed on cDNA microarray
platform.The green channel of all the arrays are common,the untreated
cell.Here follows the experiment design:
a_0hr,a_3hr,a_8hr,a_12hr,a_24hr,a_48hr,a_72hr,
b_3hr,b_8hr,b_12hr,b_24hr,b_48hr,b_72hr,
ab_3hr,ab_8hr,ab_12hr,ab_24hr,ab_48hr,ab_72hr.
A total of 19 *cDNA microarrays*.a_0hr means* *drug *a *treament *0
hours
vs. control. *And a_3hrs means drug a treatment 3 hours vs. control.So
for
drug *b *and their combination *ab*(drug a and drug b added
together).My
goal is to identify the three sets of genes,the genes differentially
expressed by drug a,the genes by drug b ,and their combination.
i am thinking about *ANOVA* ,but i am not sure whether it is correct.
Any comments,suggestions?Any R/bioconductor packages can be
used?Thanks in
advance
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